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科研成果
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| 论文题目: |
Characterization of a new Pm2 allele associated with broadspectrum powdery mildew
resistance in wheat line Subtil |
| 论文编号: |
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| 作者: |
Yuli Jin(靳玉丽), Hongxing Xu(许红星), Pengtao Ma(马朋涛), Xiaoyi Fu, Liping Song(宋丽萍), Yunfeng X(许云峰), Xiaotian Zhang(张晓天) & Diaoguo An(安调过) |
| 刊物名称: |
Scientific reports |
| 卷期页码: |
8, 475, |
| 发表年度: |
2018 |
| DOI: |
http://doi.org/10.1038/s41598-017-18827-4 |
| 摘要: |
Wheat powdery mildew is a severe disease affecting yield and quality. Host resistance was proved to be effective and environment-friendly. Wheat line Subtil is an elite germplasm resource resistant to 28 of 30 tested Bgt isolates. Genetic analysis showed that the powdery mildew resistance in Subtil was conferred by a single dominant gene, temporarily designated PmSub. Using bulked segregant analysis, PmSub was mapped to chromosome arm 5DS, and flanked by the markers Bwm16 and Cfd81/Bwm21 at 5.0 and 0.9 cM, respectively. Allelism tests further confirmed PmSub was allelic with documented Pm2 alleles. Then, homologous sequences of Pm2a related sequence was cloned from Subtil and Chinese Spring. It was completely identical to the reported Pm2a sequence, but significantly different from that of Chinese Spring. A marker SWGI067 was developed based on the sequence divergence of homologous sequence in Subtil and Chinese Spring. SWGI067 was closely linked to PmSub, indicating that the gene PmSub itself was different from the cloned Pm2a related sequence. Meanwhile, Subtil produced significantly different reaction pattern compared with other genotypes with Pm genes at or near Pm2 locus. Therefore, PmSub was most likely a new allele of Pm2. PmSub has opportunities for markerassisted selecting for high-efficiency wheat improvement. |
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